製品の概要

  • 製品名Caspase 9 Assay Kit (Fluorometric)
    Caspase 9 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • 別名
    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Caspase 9 Assay Kit (Fluorometric) 画像

  • Caspase 9 activity in Jurkat cells following 24 hour exposure to 50 ng x mL-1 anti-Fas Ab (MBL), 2 uM or 10 uM Camptothecin (CPT; ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (Zvad; ab120487). Sample input 5-25 uL, background signal subtracted, , duplicates; +/- SD.

プロトコール

Caspase 9 Assay Kit (Fluorometric) (ab65607) 使用論文

This product has been referenced in:
  • Lee YJ  et al. Apoptotic cell instillation after bleomycin attenuates lung injury through hepatocyte growth factor induction. Eur Respir J 40:424-35 (2012). Read more (PubMed: 22441736) »

See 1 Publication for this product

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Thank you for calling Abcam earlier today.

I talked to the lab and they confirmed that you will have to read the signal with the wavelengths for excitation set at 400 nm and emission set at 505 nm. Youonly take one reading.

Hope this ...

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