製品の概要

  • 製品名Anti-Caspase-9 antibody
    Caspase-9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-9
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-9 aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P55211
    (Peptide available as ab100814, ab100814)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: A431 - EGF-treated; HeLa; HepG2; HEK293; Jurkat.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50, 46 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 発生段階Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻訳後修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody 画像

  • All lanes : Anti-Caspase-9 antibody (ab90236) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF-treated
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46,50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
    We hypothesize that the 50 and 46 kDa bands represent the pro and mature forms of Caspase 9, respectively. Abcam welcomes customer feedback.

Anti-Caspase-9 antibody (ab90236) 使用論文

ab90236 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab90236.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"