製品の概要

  • 製品名Anti-Caspase-9 antibody
    Caspase-9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-9
  • 特異性Detects bands of ~46-50kDa (proform) and ~35/37kDa, ~18kDa, ~10kDa (processed) by Western blot.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Human, Monkey
  • 免疫原

    Recombinant fragment corresponding to Human Caspase-9 (N terminal). procaspase-9 region
    Database link: P55211

  • ポジティブ・コントロール
    • HeLa Cell Lysate (Heat Shocked); Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 発生段階Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻訳後修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody 画像

  • All lanes : Anti-Caspase-9 antibody (ab69514) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : apoptosis-induced Jurkat cell lysate


    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,40 kDa. We are unsure as to the identity of these extra bands.

Anti-Caspase-9 antibody (ab69514) 使用論文

ab69514 has not yet been referenced specifically in any publications.

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Thank you for providing the completed questionnaire form. I will be happy to help you resolve any issues you are having with ab44289. I just have a few comments: 1. It seems for storage you have diluted ab44289 50x in PBS before freezing?...

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