製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Caspase-9
  • 由来種
    Rabbit
  • 特異性
    ab52298 detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp 353.
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab171520)

  • ポジティブ・コントロール
    • Human lung carcinoma tissue.
  • 特記事項
    ab52298 is cleaved at Asp353

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20520761
IHC-Fr Use at an assay dependent concentration. PubMed: 21765948
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520).

ターゲット情報

  • 機能
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 組織特異性
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 配列類似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 発生段階
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻訳後修飾
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

画像

  • ab52298 at 1/50 dilution staining Caspase 9 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Caspase-9 antibody (ab52298) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate with Blocked with synthesized peptide

    Predicted band size: 46 kDa

  • ab52298 staining Caspase 9 - Cleaved Asp353 in mouse primary C4-HI cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 10% formalin for 20 minutes at room temperature and incubated overnight at 4°C with the primary antibody. The corresponding secondary FITC-conjugated antibody was used at 1/100 dilution and incubated for 1 hour at room temperature. Nuclei were stained red with propidium iodide.
  • Immunohistochemical analysis of frozen retina tissue, taken from wild-type (top) or S334ter rhodopsin transgenic rats (bottom).

    Caspase 9 - Cleaved Asp353 was stained using ab52298 at 1/100 dilution. An AlexaFluor® 488-conjugated IgG was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Xu T  et al. Oridonin enhancesin vitroanticancer effects of lentinan in SMMC-7721 human hepatoma cells through apoptotic genes. Exp Ther Med 14:5129-5134 (2017). Read more (PubMed: 29201227) »
  • Cheng Z  et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). WB . Read more (PubMed: 29104666) »

See all 12 Publications for this product

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