製品の概要

  • 製品名Anti-Caspase-9 antibody
    Caspase-9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-9
  • 特異性ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab38268)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab25758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).Can be blocked with Mouse Caspase-9 peptide (ab38268).

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 発生段階Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻訳後修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody 画像

  • Anti-Caspase-9 antibody (ab25758) + mouse intestine tissue lysate

    Predicted band size : 46 kDa
    The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

Anti-Caspase-9 antibody (ab25758) 使用論文

This product has been referenced in:
  • Gao J  et al. Emodin suppresses hyperglycemia-induced proliferation and fibronectin expression in mesangial cells via inhibiting cFLIP. PLoS One 9:e93588 (2014). WB . Read more (PubMed: 24691542) »
  • Zhao Y  et al. A novel wnt regulatory axis in endometrioid endometrial cancer. Cancer Res 74:5103-17 (2014). Read more (PubMed: 25085246) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

As this is an exceptional free of charge replacement, I will process the order. Could you please confirm that you would like a free of charge replacement with ab25758 and not ab133520 (rabbit monoclonal I recomme...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Human breast tumour)
Specification Human breast tumour
Treatment 2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
Gel Running Conditions Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Penelope Ottewell

Verified customer

投稿 Jul 04 2008

I can confirm that the antibody does not detect any other caspases. Please do not hesitate to contact us if you require further information,

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"