製品の概要

  • 製品名Caspase 8 Assay Kit (Fluorometric)
    Caspase 8 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 8 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 8 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 細胞内局在Cytoplasmic
  • 別名
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab39534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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Caspase 8 Assay Kit (Fluorometric) (ab39534) 使用論文

ab39534 has not yet been referenced specifically in any publications.

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