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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-8 aa 200-300 (internal sequence).
Database link: Q14790
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Our Abpromise guarantee covers the use of ab108333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended.|
|ICC/IF||1/100 - 1/250.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase-8 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108333 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108333 was shown to specifically react with Caspase-8 when Caspase-8 knockout samples were used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. ab108333 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab108333, at 1/100 dilution, staining Caspase-8 in paraffin-embedded human tonsil tissue by Immunohistochemistry.
ab108333, at 1/100 dilution, staining Caspase-8 in HeLa cells by Immunofluorescence.
ab108333, at 1/100 dilution, staining Caspase-8 in paraffin-embedded human hepatocellular carcinoma by Immunohistochemistry.
ab108333 (1/200) staining Caspase-8 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton-X100 (in PBS) and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.