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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-8 aa 1-100 (N terminal).
Database link: Q14790
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab32125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase-8 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32125 was shown to recognize Caspase-8 when Caspase-8 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. ab32125 and ab8226 (loading control to beta actin) were diluted 1/3000 and 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"