製品の概要

  • 製品名Anti-Caspase-8 antibody
    Caspase-8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-8
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Monkey
  • 免疫原

    Recombinant full length protein corresponding to Human Caspase-8.
    Database link: Q14790

  • ポジティブ・コントロール
    • Stomach.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.

ターゲット情報

  • 機能Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • 組織特異性Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • 関連疾患Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • ドメインIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • 翻訳後修飾Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS2B antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch-5 antibody
    • Apoptotic protease Mch5 antibody
    • CAP4 antibody
    • CASP-8 antibody
    • CASP8 antibody
    • CASP8_HUMAN antibody
    • Caspase 8 antibody
    • Caspase 8 apoptosis related cysteine peptidase antibody
    • Caspase-8 subunit p10 antibody
    • CED 3 antibody
    • FADD Like ICE antibody
    • FADD-homologous ICE/CED-3-like protease antibody
    • FADD-like ICE antibody
    • FLICE antibody
    • FLJ17672 antibody
    • ICE-like apoptotic protease 5 antibody
    • MACH alpha 1/2/3 protein antibody
    • MACH antibody
    • MACH beta 1/2/3/4 protein antibody
    • MCH5 antibody
    • MGC78473 antibody
    • MORT1 associated ced 3 homolog antibody
    • MORT1-associated CED-3 homolog antibody
    • OTTHUMP00000163717 antibody
    • OTTHUMP00000163720 antibody
    • OTTHUMP00000163724 antibody
    • OTTHUMP00000163725 antibody
    • OTTHUMP00000165062 antibody
    • OTTHUMP00000165063 antibody
    • OTTHUMP00000165064 antibody
    • OTTHUMP00000206552 antibody
    • OTTHUMP00000206582 antibody
    see all

Anti-Caspase-8 antibody 画像

  • ab4052 staining Caspase-8 in human kidney tissue section by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue  underwent fixation in paraformaldehyde, heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0, permeabilization in 0.1% Triton X-100 prior to blocking in 10% serum for 20 minutes at 22°C. The primary antibody, diluted 1/50 in PBS with 2% BSA and incubated with sample, for 45 minutes at 22°C. An Avidin-peroxidase conjugated horse polyclonal to rabbit Ig, was used undiluted as the secondary antibody.

    See Abreview

  • ab4052 staining Caspase-8 in formalin-fixed, paraffin-embedded Human stomach tissue by Immunohistochemistry.

Anti-Caspase-8 antibody (ab4052) 使用論文

This product has been referenced in:
  • Negrette-Guzmán M  et al. Sulforaphane attenuates gentamicin-induced nephrotoxicity: role of mitochondrial protection. Evid Based Complement Alternat Med 2013:135314 (2013). Read more (PubMed: 23662110) »
  • Sansanwal P  et al. Caspase-4 may play a role in loss of proximal tubules and renal injury in nephropathic cystinosis. Pediatr Nephrol : (2009). IHC-P ; Human . Read more (PubMed: 19705160) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

I'm afraid we are unable to determine if the antibody is specific to the active form only of caspase 8 or the pro-form as it has only been tested in IHC. We also haven't tested it in staining cells (ICC/IF). Most...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer, pH 6.0
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Dr. B Goilav

Verified customer

投稿 Dec 18 2008

I'm afraid we are unable to determine if the antibody is specific to the active form only of caspase 8, as it has only been tested in IHC. I'm sorry I cannot be of further assistance on this occasion.

I can confirm that the customer's protocol is adequate and should work. However, we have the following suggestions: 1. Try using another tissue besides liver. We recommend stomach. 2. The customer is using home brewed detection system. The ...

Read More

Thank you for those further protocol details, I would like to recommend diluting the blocking serum, primary and secondary antibodies in PBST containing triton x100 (0.3%v/v) to promote permeabilization of the tissue and enable better penetration of th...

Read More

I'm very sorry to hear you are having problems with ab4052, thank you for taking the time to fill in the online questionnaire. Could you please tell me: -how the antibody was stored? - how long the antigen retrieval step was? - if you have done a perme...

Read More

Thank you for your enquiry. This antibody recognizes the full length inactive proenzyme (55 kDa). While this antibody has also been shown to detect the processed forms of Caspase 8 (which associate to form the active enzyme), its specific reaction wit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"