製品の概要

  • 製品名Caspase 8 (active) Red Staining Kit
    Caspase 8 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 8 (active) Red Staining Kit provides a convenient means for detecting activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor IETD-FMK conjugated to sulfo-rhodamine (Red-IETD-FMK) as the fluorescent in situ marker. Red-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 8 in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of caspases plays a central role in apoptosis.

  • アプリケーション適用あり: FM, Functional Studies, Flow Cytmore details

製品の特性

  • 関連性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 細胞内局在Cytoplasmic
  • 別名
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab65618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

プロトコール

Caspase 8 (active) Red Staining Kit (ab65618) 使用論文

ab65618 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab65618.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"