製品の概要

  • 製品名Caspase 8 (active) FITC Staining Kit
    Caspase 8 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Caspase 8 (active) FITC Staining Kit (ab65614) provides a convenient means for sensitive detection of activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a marker. FITC-IETD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 8 in apoptotic cells. The FITC label allows detection of activated caspase-8 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of caspases plays a central role in apoptosis.

  • アプリケーション適用あり: FM, Functional Studies, Flow Cytmore details

製品の特性

  • 関連性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 細胞内局在Cytoplasmic
  • 別名
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab65614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Caspase 8 (active) FITC Staining Kit 画像

  • Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.

  • Active caspase 8 in Jurkat cells following four hours exposure to 50 ng/mL anti-Fas Ab (αFas) (MBL), five hours with 10 μg/mL cyclohexamide (CHX) (ab120093), or one hour pretreatment with CHX followed by four hours with αFas. Background signal subtracted, duplicates; +/- SD.

  • Caspase 8 activation of Hela (left) and SiHa (right) cells after in vitro treatment with pentoxylline (PTX) or cisplatin (CIS) either alone or in combination. Results represent the mean ± SD of three independent experiments carried out in triplicate. (*) p<0.001 vs CTL.

    Image obtained from Hernandez-Flores G et al; BMC Cancer, 2011 Nov 11; 11:483

プロトコール

Caspase 8 (active) FITC Staining Kit (ab65614) 使用論文

This product has been referenced in:
  • Hernandez-Flores G  et al. Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence. BMC Cancer 11:483 (2011). Read more (PubMed: 22074157) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. 1.) You may use Caspase-8 negative cells (cells that were not stimulated to induce Caspase-8) for your negative control in Flow Cytometry. 2.) For a viability dye, you may try using PI but this will need to be optimi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"