Caspase 7 Inhibitor Drug Detection Kit (ab102495)

製品の概要

  • 製品名Caspase 7 Inhibitor Drug Detection Kit
  • 検出方法Fluorescent
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Abcam's Caspase 7 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 特記事項

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
  • 別名
    • CASP7 inhibitor

アプリケーション

Our Abpromise guarantee covers the use of ab102495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

The assay was tested useing approxamatly 1-5 x 10^6 cells.

For the inhibitor, the same concentration range as mentioned for the control inhibitor (1 ul of 2 mM solution) should be used.

Caspase 7 Inhibitor Drug Detection Kit 画像

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase substrate and following Caspase 7 Inhibitor Drug Detection Kit (ab102495) protocol. Lanes:

    1.- Background Control: no Caspase 7, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 7, +z-VAD-FMK

    3.- Positive Control: + Caspase 7, no z-VAD-FMK

    4.- Caspase 7 + 2.5µM z-VAD-FMK

    5.- Caspase 7 + 10µM z-VAD-FMK

    6.- Caspase 7 + 40µM z-VAD-FMK

プロトコール

Caspase 7 Inhibitor Drug Detection Kit (ab102495) 使用論文

ab102495 has not yet been referenced specifically in any publications.

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I consulted the lab to make sure I can give you the information needed. The information in the protocol about sample preparation is very brief.

Under step one in the protocol for the kit you wi...

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