Anti-Caspase-7 抗体 (ab52297)

製品の概要

  • 製品名Anti-Caspase-7 antibody
    Caspase-7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-7
  • 特異性ab52297 detects endogenous levels of fragment of activated Caspase-7 resulting from cleavage adjacent to Asp198.
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-7.
    Database link: P55210

  • ポジティブ・コントロール
    • Human lung carcinoma tissue.
  • 特記事項

    ab52297 is Cleaved at Asp198

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • 組織特異性Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Anti-Caspase-7 antibody 画像

  • ab52297 at 1:50 dilution staining Caspase-7 in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    ab52297 at 1/50 dilution staining Caspase 7 in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-Caspase-7 antibody (ab52297) 使用論文

ab52297 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal carcinoma)
Specification Colorectal carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Mr. Philip Noble

Verified customer

投稿 Jun 10 2011

There is no antibody specific only for the activated form. As the antibody will recognize the same sequence despite whether it is in the pro-form or processed form. Pro-caspase antibodies are specific as the active version will not have the specific p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"