Anti-Caspase-6 (phospho S257) 抗体 (ab63440)

製品の概要

  • 製品名Anti-Caspase-6 (phospho S257) antibody
    Caspase-6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-6 (phospho S257)
  • 特異性ab63440 detects endogenous levels of Caspase-6 only when phosphorylated at serine 257.
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-6.
    Database link: P55212

  • ポジティブ・コントロール
    • Human colon carcinoma tissue, HuvEc cells and 293 cell extracts treated with Etoposide (25uM, 60min).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Anti-Caspase-6 (phospho S257) antibody 画像

  • ab63440 at 1:500 - 1:1000 dilution staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • ab63440 at 1:50 - 1:100 dilution staining Caspase-6 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

  • All lanes : Anti-Caspase-6 (phospho S257) antibody (ab63440) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Etoposide (25uM, 60min)
    Lane 2 : 293 cell extracts treated with Etoposide (25uM, 60min) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading is generally 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

Anti-Caspase-6 (phospho S257) antibody (ab63440) 使用論文

ab63440 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"