製品の概要

  • 製品名Caspase 6 Assay Kit (Fluorometric)
    Caspase 6 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 6 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 6 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
  • 細胞内局在Cytoplasmic
  • 別名
    • Apoptotic Protease MCH2
    • CASP6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6 precursor
    • MCH2
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab39707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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Caspase 6 Assay Kit (Fluorometric) (ab39707) 使用論文

ab39707 has not yet been referenced specifically in any publications.

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