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RabMAb

Anti-Caspase-6 抗体 [EPR4405] (ab108335)

製品の概要

  • 製品名
    Anti-Caspase-6 antibody [EPR4405]
    Caspase-6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4405] to Caspase-6
  • 特異性
    ab108335 detects both the full length and cleaved forms of Caspase-6.
  • アプリケーション
    適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 150-250.
    Database link: P55212

  • ポジティブ・コントロール
    • Jurkat cells and cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • 配列類似性
      Belongs to the peptidase C14A family.
    • 翻訳後修飾
      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    画像

    • All lanes : Anti-Caspase-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat + staurosporine cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 33 kDa
    • ab108335, at 1:10 dilution, staining Caspase-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).

    参考文献

    This product has been referenced in:
    • Ma J  et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). Read more (PubMed: 27167569) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Mouse Cell (ledyig cells)
    Specification
    ledyig cells
    Gating Strategy
    RLF positive cells
    Preparation
    Cell harvesting/tissue preparation method: Tubules from testes were incubated for 15–20 min at 35¯C in a shaking water bath at 80 oscillations (osc)/min , then layered over 40 mL 5% Percoll and leydig cells were isolated from top of the percoll
    Sample buffer: 0.1% PBS BSA
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    投稿 Dec 04 2013

    Application
    Flow Cytometry
    Fixation
    Methanol
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Rat Cell (breast cells)
    Specification
    breast cells
    Gating Strategy
    cd1d1 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: tissue was treated with collagenase to make single cell suspension
    Sample buffer: 0.1% PBS BSA
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    投稿 Dec 04 2013

    Application
    Flow Cytometry
    Fixation
    Methanol
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Human Cell (MCF7)
    Specification
    MCF7
    Gating Strategy
    CD44 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: treated with collagenase and trypsin to make single cell suspension
    Sample buffer: 0.1% PBS BSA
    Username

    Abcam user community

    Verified customer

    投稿 Dec 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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