Anti-Caspase-6/CASP-6 抗体 [EP1325Y] (ab52951)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1325Y] to Caspase-6/CASP-6
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Caspase-6/CASP-6 antibody [EP1325Y]
Caspase-6/CASP-6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1325Y] to Caspase-6/CASP-6 -
由来種
Rabbit -
特異性
ab52951 should recognize the pro-form of Caspase-6/CASP-6.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Caspase-6/CASP-6 aa 150-250. The exact sequence is proprietary.
Database link: P55212 -
ポジティブ・コントロール
- MCF-7 cell lysate, human breast carcinoma and HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1325Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52951の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/20000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval recommended |
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/20000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval recommended |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 839 Human
- Entrez Gene: 12368 Mouse
- Entrez Gene: 83584 Rat
- Omim: 601532 Human
- SwissProt: P55212 Human
- SwissProt: O08738 Mouse
- SwissProt: O35397 Rat
- Unigene: 654616 Human
see all -
別名
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
see all
画像
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Anti-Caspase-6/CASP-6 antibody [EP1325Y] (ab52951) at 1/20000 dilution + MCF-7 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab52951 at 1:100 dilution staining Caspase-6/CASP-6 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab52951 at 1:100 dilution staining Caspase-6/CASP-6 in HeLa cells by Immunofluorescence.
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Overlay histogram showing Hela cells stained with ab52951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab52951 は論文での使用が確認できていません。