Anti-Caspase-6 抗体 [EP1325Y] (ab52951)

製品の概要

  • 製品名Anti-Caspase-6 antibody [EP1325Y]
    Caspase-6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1325Y] to Caspase-6
  • 特異性ab52951 should recognize the pro-form of Caspase-6.
  • アプリケーション適用あり: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 150-250.
    Database link: P55212

  • ポジティブ・コントロール
    • MCF-7 cell lysate, human breast carcinoma and HeLa cells.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/50.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250.

Antigen retrieval recommended

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Anti-Caspase-6 antibody [EP1325Y] 画像

  • ab52951 at 1:100 dilution staining Caspase-6 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.

  • ab52951 at 1:100 dilution staining Caspase-6 in HeLa cells by Immunofluorescence.

  • Overlay histogram showing Hela cells stained with ab52951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Caspase-6 antibody [EP1325Y] (ab52951) at 1/20000 dilution + MCF-7 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

Anti-Caspase-6 antibody [EP1325Y] (ab52951) 使用論文

ab52951 has not yet been referenced specifically in any publications.

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