製品の概要

  • 製品名Anti-Caspase-6 antibody [E180]
    Caspase-6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E180] to Caspase-6
  • 特異性This antibody detects the pro form and the p11 subunit of human Caspase-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 250-350 (C terminal).
    Database link: P55212

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • 配列類似性Belongs to the peptidase C14A family.
    • 翻訳後修飾Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Anti-Caspase-6 antibody [E180] 画像

    • Ab32366, at a 1:50 dilution, staining Caspase-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.

    • Ab32366, at a 1:100 dilution, staining Caspase-6 in Hela cells by Immunocytochemistry/Immunofluorescence.

    • All lanes : Anti-Caspase-6 antibody [E180] (ab32366) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate treated with Camptothecin.


      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Anti-Caspase-6 antibody [E180] (ab32366) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

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