製品の概要

  • 製品名Caspase 5 Inhibitor Drug Detection Kit
  • 検出方法Fluorescent
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Abcam's Caspase 5 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate WEHDD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 5 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 5 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
  • 別名
    • CASP5 inhibitor

アプリケーション

Our Abpromise guarantee covers the use of ab102493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Caspase 5 Inhibitor Drug Detection Kit 画像

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using WEHDD-AFC as caspase substrate and following Caspase 5 Inhibitor Drug Detection Kit (ab102493) protocol. Lanes:

    1.- Background Control: no Caspase 5, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 5, +z-VAD-FMK

    3.- Positive Control: + Caspase 5, no z-VAD-FMK

    4.- Caspase 5 + 2.5µM z-VAD-FMK

    5.- Caspase 5 + 10µM z-VAD-FMK

    6.- Caspase 5 + 40µM z-VAD-FMK

プロトコール

Caspase 5 Inhibitor Drug Detection Kit (ab102493) 使用論文

ab102493 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab102493.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"