製品の概要

  • 製品名Caspase 5 Assay Kit (Colorimetric)
    Caspase 5 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 5 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate WEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Caspase 5 belongs to the Ced3/ICE family of cysteine proteases. Caspase 5 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues, leading to p20 and p10 subunits that dimerize to form the active enzyme. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
  • 別名
    • CASP 5
    • CASP5
    • Caspase 5 apoptosis related cysteine peptidase
    • Caspase5
    • ICE(rel) III
    • ICE(rel)III
    • ICEREL III
    • ICERELIII
    • ICH 3
    • ICH 3 protease
    • ICH3
    • MGC141966
    • TY protease
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab65661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

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Caspase 5 Assay Kit (Colorimetric) (ab65661) 使用論文

ab65661 has not yet been referenced specifically in any publications.

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