Anti-Caspase 5 抗体 [CAS5] (ab10448)

製品の概要

  • 製品名Anti-Caspase 5 antibody [CAS5]
    Caspase 5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CAS5] to Caspase 5
  • アプリケーション適用あり: ELISA, WB, Double Immunodiffusionmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    GEKHGELWVR

    conjugated to KLH, corresponding to amino acids 300-311 of Human Caspase 5.

  • エピトープThe epitope recognized by the antibody resides within amino acids 300-311 of human Caspase 5 molecule.
  • ポジティブ・コントロール
    • Whole extract of HeLa cells activated with lipopolysaccharide (LPS).
  • 特記事項For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名CAS5
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
Double Immunodiffusion
  • 追加情報DID: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 5 - 10 µg/ml (determined by using a whole extract of HeLa cells activated with lipopolysaccharide (LPS)). Expected bands: a doublet at approx. 42 kDa, with additional bands at approx. 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mediator of programmed cell death (apoptosis).
    • 組織特異性Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • 配列類似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 翻訳後修飾The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Anti-Caspase 5 antibody [CAS5] (ab10448) 使用論文

    ab10448 has not yet been referenced specifically in any publications.

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