製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Caspase 5
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Caspase 5.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line:HeLa.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Mediator of programmed cell death (apoptosis).
  • 組織特異性
    Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
  • 配列類似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis related cysteine protease antibody
    • CASP-5 antibody
    • CASP5 antibody
    • CASP5_HUMAN antibody
    • Caspase-5 subunit p10 antibody
    • ICE(rel)-III antibody
    • ICERELIII antibody
    • ICH 3 antibody
    • ICH 3 protease antibody
    • Protease ICH-3 antibody
    • Protease TY antibody
    • TY antibody
    • TY protease antibody
    see all

画像

  • Anti-Caspase 5 antibody (ab92354) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 24 kDa,50 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab92354 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92354 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) MCF-7 cell types.

参考文献

ab92354 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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