Rabbit polyclonal to Caspase 5
ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
A synthesized peptide derived from human Caspase 5
Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Mediator of programmed cell death (apoptosis).
Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Information by UniProt
Apoptosis related cysteine protease antibody
Western blot - Caspase 5 antibody (ab69641)
All lanes :
Anti-Caspase 5 antibody (ab69641) at 1/500 dilution
Lane 1 :
HeLa cells treated with etoposide (25uM, 24hours)
Lane 2 :
A549 cells treated with etoposide (25uM, 24hours)
Lane 3 :
HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size:
Observed band size:
26,48 kDa (
why is the actual band size different from the predicted?
Additional bands at:
26 kDa (possible cleavage fragment)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"