Anti-Caspase-5 抗体 (ab69641)
Key features and details
- Rabbit polyclonal to Caspase-5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Caspase-5 antibody
Caspase-5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-5 -
由来種
Rabbit -
特異性
ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-5.
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ポジティブ・コントロール
- Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69641の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Mediator of programmed cell death (apoptosis). -
組織特異性
Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻訳後修飾
The two subunits are derived from the precursor sequence by an autocatalytic mechanism. - Information by UniProt
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参照データベース
- Entrez Gene: 838 Human
- Omim: 602665 Human
- SwissProt: P51878 Human
- Unigene: 213327 Human
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別名
- Apoptosis related cysteine protease antibody
- CASP-5 antibody
- CASP5 antibody
see all
画像
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All lanes : Anti-Caspase-5 antibody (ab69641) at 1/500 dilution
Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
Lane 2 :
A549 cells treated with etoposide (25uM, 24hours)
Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 26,48 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible cleavage fragment)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab69641 は論文での使用が確認できていません。