製品の概要

  • 製品名Caspase 4 Assay Kit (Colorimetric)
    Caspase 4 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 4 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEVD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Caspase 4 is a member of the cysteine-aspartic acid protease (caspase) family. It is involved in the activation cascade of caspases responsible for apoptosis execution. Caspases exist as inactive proenzymes. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of large and small subunits. Caspase 4 is able to cleave and activate its own precursor protein, as well as the caspase 1 precursor. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.
  • 細胞内局在Cytoplasmic and Mitochondrial
  • 別名
    • CASP4
    • Caspase4
    • ICE(rel)II
    • ICERELII
    • ICH2
    • TX
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

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Caspase 4 Assay Kit (Colorimetric) (ab65659) 使用論文

ab65659 has not yet been referenced specifically in any publications.

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