特異性ab22687 recognises proenzyme (43 kDa) and the large subunit (19 kDa) and small (10 kDa) subunits of mature enzyme. For the mature enzyme, reactivity is strongest with the large subunit. No cross reactivity with other human caspases tested (1 to 3, 5 to 10, 14).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: 1/8000 - 1/16000. Detects bands of approximately 43, 19 and 10 kDa corresponding to the proenzyme, the large subunit and small subunits of mature enzyme, respectively (using Alkaline phosphatase-linked secondary antibody).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
組織特異性Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
配列類似性Belongs to the peptidase C14A family. Contains 1 CARD domain.
翻訳後修飾The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
Park GB et al. Endoplasmic Reticulum Stress-Mediated Apoptosis of EBV-Transformed B Cells by Cross-Linking of CD70 Is Dependent upon Generation of Reactive Oxygen Species and Activation of p38 MAPK and JNK Pathway. J Immunol185:7274-84 (2010).
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