Caspase 3 Inhibitor Drug Detection Kit (ab102491)

製品の概要

  • 製品名
    Caspase 3 Inhibitor Drug Detection Kit
    Caspase 3 キット 製品一覧
  • 検出方法
    Fluorescent
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Abcam's Caspase 3 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 特記事項

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • アプリケーション
    適用あり: Functional Studiesmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

画像

  • Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).

  • Titration of the Caspase 3 specific inhibitor Q-DEVD-OPh (ab142037) (duplicates; +/- SD).

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase 3 substrate and following Caspase 3 Inhibitor Drug Detection Kit (ab102491) protocol. Lanes:

    1.- Background Control: no Caspase 3, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 3, +z-VAD-FMK

    3.- Positive Control: + Caspase 3, no z-VAD-FMK

    4.- Caspase 3 + 2.5µM z-VAD-FMK

    5.- Caspase 3 + 10µM z-VAD-FMK

    6.- Caspase 3 + 40µM z-VAD-FMK

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参考文献

ab102491 has not yet been referenced specifically in any publications.

レビューと Q&A

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The lab advices as follows:

Onewould have to add DTT to the 2X Reaction buffer before using it for reconstitution of the Active Cappase-3.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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