製品の概要

  • 製品名Anti-Caspase-3 antibody [M377]
    Caspase-3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M377] to Caspase-3
  • アプリケーション適用あり: WB, ICC, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human Caspase-3 (N terminal).
    Database link: P42574

  • ポジティブ・コントロール
    • Human Jurkat and K562 cells. IHC-P: human spleen tissue sections
  • 特記事項Do not aliquot.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab119794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 31 kDa.
ICC 1/100.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 組織特異性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody [M377] 画像



  • Predicted band size : 31 kDa
    Lane 1 = Caspase 3 protein (Active) (ab52314) ? 20 ng. Lane 2 = Extract of Jurkat cells treated with vehicle ? 20 ug. Lane 3 = Extract of Jurkat cells treated with staurosporine (ab120056) ? 20 ug. SDS PAGE performed under reducing conditions (100 mM DTT ? Sample heated at 50?C). Primary : Lanes 1-3: Anti Caspase 3 antibody (ab119794) at 1:1000 dilution. Lanes 4-6: Anti Caspase 3 antibody (ab32351) at 1:1000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 10 min exposure. Blocking: in 5% Milk + PBS overnight at 4 C. Primary antibody: in 5% Milk + PBS for 4 hours at RT. Secondary antibody: in 5% Milk + PBS for 2 hours at RT. Predicted band size : 32 kDa (lane 2) and 17 kDa (lanes 1 and 3). Observed band size : 32 kDa (lane 2). Note: 17 kDa band not observed in lanes 1 and 3
  • Lane 1 : Anti-Caspase-3 antibody [M377] (ab119794) at 1/500 dilution
    Lane 2 : Anti-Caspase-3 antibody [M377] (ab119794) at 1/1000 dilution

    Lane 1 : Extract from Human Jurkat cells
    Lane 2 : Extract from Human Jurkat cells


    Predicted band size : 31 kDa
  • IHC image of ab119794 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Caspase-3 antibody [M377] (ab119794) 使用論文

ab119794 has not yet been referenced specifically in any publications.

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