Anti-Caspase-3 抗体 (ab90437)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB, IHC-P
- Reacts with: Human, Saccharomyces cerevisiae
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Caspase-3 antibody
Caspase-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Saccharomyces cerevisiae -
免疫原
Recombinant full length protein corresponding to Human Caspase-3 aa 1-277.
Sequence:MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELM RDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRS LTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTA PGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESF SFDATFHAKKQIPCIVSMLTKELYFYH
Database link: P42574 -
ポジティブ・コントロール
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Affinity Purification -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab90437の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (7) |
1/1000. Predicted molecular weight: 32 kDa.
A lower molecular weight band refers to a proteolytic cleavage product of caspase-3. |
IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 32 kDa. A lower molecular weight band refers to a proteolytic cleavage product of caspase-3. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
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別名
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
画像
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All lanes : Anti-Caspase-3 antibody (ab90437) at 1/1000 dilution
Lane 1 : Mouse hepatocytes whole cell lysate poly(I:C) treated - 0 hours
Lane 2 : Mouse hepatocytes whole cell lysate poly(I:C) treated - 12 hours
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 20 secondsBlocking with 5% milk for 1 hour at 23°C.
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Immunohistochemistry analysis of human spleen tissue stained with Caspase-3, pAb at 10µg/ml.
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All lanes : Anti-Caspase-3 antibody (ab90437) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cells treated with staurosporine
Lane 3 : MCF-7 cell lysate (negative control)
Developed using the ECL technique.
Predicted band size: 32 kDa
Additional bands at: ~18 kDa (possible cleavage fragment), ~20 kDa (possible cleavage fragment)Western blot analysis of Caspase-3 pAb.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (103)
ab90437 は 103 報の論文で使用されています。
- Patel S et al. Chondroprotective effects of multiple PRP injections in osteoarthritis by apoptosis regulation and increased aggrecan synthesis- Immunohistochemistry based Guinea pig study. J Clin Orthop Trauma 25:101762 (2022). PubMed: 35070686
- Zhang T et al. Hsa_circ_0005100 regulates tumorigenicity of colorectal carcinoma via miR-145-5p/MACC1 axis. J Clin Lab Anal 36:e24533 (2022). PubMed: 35766445
- Xu W et al. PIK3CB promotes oesophageal cancer proliferation through the PI3K/AKT/mTOR signalling axis. Cell Biol Int 46:1399-1408 (2022). PubMed: 35842767
- Zhang G et al. KDELR2 knockdown synergizes with temozolomide to induce glioma cell apoptosis through the CHOP and JNK/p38 pathways. Transl Cancer Res 10:3491-3506 (2021). PubMed: 35116653
- Gan YR et al. Dickkopf-1/cysteine-rich angiogenic inducer 61 axis mediates palmitic acid-induced inflammation and apoptosis of vascular endothelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33300071