製品の概要

  • 製品名Anti-Caspase-3 antibody
    Caspase-3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-3
  • 特異性ab52293 detects endogenous levels of fragment of activated Caspase-3 resulting from cleavage adjacent to Asp175.
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-3 aa 140-180 (internal sequence).
    Database link: P42574

  • ポジティブ・コントロール
    • IHC: Human lung carcinoma tissue, ICC/IF: HeLa cell line
  • 特記事項


    This is a Caspase 3 (cleaved-Asp175) antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 17, 32 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/20000.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 組織特異性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody 画像

  • Lane 1 : Anti-Caspase-3 antibody (ab52293) (Extracts from 293 cells treated with Etoposide (25 microM, 60 mins))
    Lane 2 : Anti-Caspase-3 antibody (ab52293) (Extracts from 293 cells treated with Etoposide (25 microM, 60 mins) and the CI-peptide)

    Lane 1 : As above
    Lane 2 : As above


    Predicted band size : 17, 32 kDa
  • ICC/IF image of ab52293 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52293, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, anti-rabbit DyLight® 488 used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC- paraffin-embedded human lung carcinoma tissue using ab52293. The image on the right is treated with the synthesized peptide.

Anti-Caspase-3 antibody (ab52293) 使用論文

This product has been referenced in:
  • Costa B  et al. Human glioblastoma multiforme: p53 reactivation by a novel MDM2 inhibitor. PLoS One 8:e72281 (2013). IHC-P . Read more (PubMed: 23977270) »
  • Kou X  et al. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells. BMC Cancer 13:438 (2013). WB ; Human . Read more (PubMed: 24066693) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Human skeletal muscle)
Permeabilization No
Specification Human skeletal muscle
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Acetone
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投稿 Jun 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Mouse Tissue sections (Tumor)
Specification Tumor
Permeabilization No
Fixative Formaldehyde
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投稿 Jul 11 2014

The labels on the vial will sometimes be truncated due to space and printing limitations. The product which you have received is the same as described on the datasheet for ab52293. The expanded product name references the ability of this antibody to re...

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Merci de nous avoir contactés.

Le kit ELISA ab100646 est en effet approprié pour le dosage du TGF-alpha humain contenu dans les supernageants de cultures cellulaires, urine, sérum et plasma.

Pour les anti-Histone ...

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Thank you for your email and letting us know that the IHC-P image was not showing correctly.

This is now fixed. Please review the datasheet.

http://www.abcam.com/index.html?datasheet=52293 (or use the following: http://www.abcam.com...

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Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"