製品の概要

  • 製品名Anti-Caspase-3 antibody
    Caspase-3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-3
  • 特異性ab52181 detects endogenous levels of fragment of activated Caspase-3 resulting from cleavage adjacent to Asp175.
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-3 aa 126-175.
    Database link: P42574

  • ポジティブ・コントロール
    • Human lung carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19443712
IHC-P 1/50 - 1/100.
ELISA 1/20000.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 組織特異性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody 画像

  • ab52181 at 1/50 dilution staining Caspase-3 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    ab52181 at 1/50 dilution staining Caspase 3 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-Caspase-3 antibody (ab52181) 使用論文

This product has been referenced in:
  • Ding X  et al. Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50:4957-66 (2009). ICC/IF . Read more (PubMed: 19443712) »
  • Ding X  et al. Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50:4957-66 (2009). ICC/IF . Read more (PubMed: 19443712) »

See all 2 Publications for this product

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To answer your question:
If the sample of isolated RNA contains activated caspase 3 of human, mouse or rat origin, the antibody would detect it usingone of the tested applications: ICC/IF, IHC-P, ELISA - giv...

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For primary antibodies, we can provide Certificate of Compliance (CoC) instead of Certificate of Analysis. Please find enclosed the requested document fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (IEC-6)
Specification IEC-6
Fixative Formaldehyde
Permeabilization Yes - 0.1% TX
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Verified customer

投稿 Feb 13 2012

Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"