製品の概要

  • 製品名Anti-Caspase-3 antibody
    Caspase-3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-3
  • 特異性ab123114 recognizes the end of the active form of human caspase-3, p20/p17 subunit but does not recognize the proenzyme p32.
  • アプリケーション適用あり: WB, ELISA, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-3 aa 170-175.
    Sequence:

    CGIETD


    Database link: P42574

  • ポジティブ・コントロール
    • NT2 neurons infected with adenovirus vector expressing ß-galactosidase or APP

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. Predicted molecular weight: 17 kDa.
ELISA Use at an assay dependent dilution.
ICC 1/500 - 1/1000.

ターゲット情報

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 組織特異性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody 画像

  • NT2 neurons (neurally differentiated Human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing beta-galactosidase (upper panel) or APP (lower panel), fixed 48 h later, and stained for the N-terminus of APP or beta-gal, chromosomal DNA (Hoechst), and activated caspase-3 subunits using ab123114 at a dilution of 1/500.

Anti-Caspase-3 antibody (ab123114) 使用論文

This product has been referenced in:
  • Nishimura I  et al. Upregulation and antiapoptotic role of endogenous Alzheimer amyloid precursor protein in dorsal root ganglion neurons. Exp Cell Res 286:241-51 (2003). Read more (PubMed: 12749853) »
  • Nishimura I  et al. Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ 9:199-208 (2002). Read more (PubMed: 11840170) »

See all 2 Publications for this product

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