製品の概要

  • 製品名Caspase 2 Inhibitor Drug Detection Kit
    Caspase 2 キット 製品一覧
  • 検出方法Fluorescent
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Abcam's Caspase 2 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 特記事項

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 組織特異性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 別名
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase-2 subunit p12
    • Caspase2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD-2
    • NEDD2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab102490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Caspase 2 Inhibitor Drug Detection Kit 画像

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using VDVAD-AFC as caspase 2 substrate and following Caspase 2 Inhibitor Drug Detection Kit (ab102490) protocol. Lanes:

    1.- Background Control: no Caspase 2, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 2, +z-VAD-FMK

    3.- Positive Control: + Caspase 2, no z-VAD-FMK

    4.- Caspase 2 + 2.5µM z-VAD-FMK

    5.- Caspase 2 + 10µM z-VAD-FMK

    6.- Caspase 2 + 40µM z-VAD-FMK

プロトコール

Caspase 2 Inhibitor Drug Detection Kit (ab102490) 使用論文

ab102490 has not yet been referenced specifically in any publications.

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