製品の概要

  • 製品名Caspase 2 Assay Kit (Fluorometric)
    Caspase 2 キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 2 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 2 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 組織特異性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 別名
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase-2 subunit p12
    • Caspase2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD-2
    • NEDD2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab39794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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Caspase 2 Assay Kit (Fluorometric) (ab39794) 使用論文

ab39794 has not yet been referenced specifically in any publications.

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