遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Caspase-2 抗体 [Y154] (ab32021)

製品の概要

  • 製品名
    Anti-Caspase-2 antibody [Y154]
    Caspase-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y154] to Caspase-2
  • 特異性
    This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-2 aa 250-350 (C terminal).
    Database link: P42575

  • ポジティブ・コントロール
    • Jurkat cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).

ターゲット情報

  • 機能
    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 組織特異性
    Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 配列類似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾
    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CASP 2 antibody
    • CASP-2 antibody
    • Casp2 antibody
    • CASP2_HUMAN antibody
    • Caspase 2 antibody
    • Caspase 2 apoptosis related cysteine peptidase antibody
    • Caspase-2 subunit p12 antibody
    • Caspase2 antibody
    • ICH 1 antibody
    • ICH 1 protease antibody
    • ICH 1L antibody
    • ICH1 antibody
    • ICH1 protease antibody
    • ICH1L antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 2 antibody
    • PPP1R57 antibody
    • Protease ICH-1 antibody
    • Protein phosphatase 1 regulatory subunit 57 antibody
    see all

画像



  • Predicted band size : 49 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa (possible cleavage fragment).

参考文献

This product has been referenced in:
  • Majumder P  et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). Read more (PubMed: 28360125) »
  • Shen Q  et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). Read more (PubMed: 25360218) »

See all 5 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab32021.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録