Caspase 2 (active) FITC Staining Kit (ab65612)
製品の概要
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製品名Caspase 2 (active) FITC Staining Kit
Caspase 2 キット 製品一覧 -
サンプルの種類Adherent cells, Suspension cells
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アッセイタイプEnzyme activity
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全工程の試験時間2h 00m
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製品の概要
Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Visit our FAQs page for tips and troubleshooting. -
特記事項
Activation of caspases plays a central role in apoptosis.
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アプリケーション適用あり: FM, Functional Studies, Flow Cytmore details
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 100 tests FITC-VDVAD-FMK 1 x 100µl Wash Buffer 2 x 100ml Z-VAD-FMK 1 x 10µl -
研究分野
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機能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
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組織特異性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
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配列類似性Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻訳後修飾The mature protease can process its own propeptide, but not that of other caspases.
- Information by UniProt
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別名
- CASP-2
- Casp2
- CASP2_HUMAN
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関連製品
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アプリケーション
Our Abpromise guarantee covers the use of ab65612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| FM | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent dilution. | |
| Flow Cyt | Use at an assay dependent dilution. |
プロトコール
参考文献
ab65612 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"