Caspase 2 (active) FITC Staining Kit (ab65612)

製品の概要

  • 製品名
    Caspase 2 (active) FITC Staining Kit
    Caspase 2 キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of caspases plays a central role in apoptosis.

  • アプリケーション
    適用あり: FM, Functional Studies, Flow Cytmore details

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

プロトコール

参考文献

ab65612 has not yet been referenced specifically in any publications.

レビューと Q&A

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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