製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Caspase-14
  • 特異性
    This antibody is specific for Caspase-14.
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-14 (C terminal).
    Database link: P31944
    (Peptide available as ab72738)

  • ポジティブ・コントロール
    • Jurkat cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2 and HeLa.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    Caspase-14 Antibody is affinity chromatography purified via peptide column.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Caspase-14 peptide (ab72738).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.
  • 組織特異性
    Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.
  • 配列類似性
    Belongs to the peptidase C14A family.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis related cysteine protease antibody
    • CASP 14 antibody
    • CASP-14 antibody
    • CASP14 antibody
    • Caspase 14 apoptosis related cysteine protease antibody
    • Caspase 14 precursor antibody
    • Caspase-14 subunit p10 antibody
    • Caspase14 antibody
    • CASPE_HUMAN antibody
    • MGC119078 antibody
    • MGC119079 antibody
    • MICE antibody
    • Mini ICE antibody
    see all

Anti-Caspase-14 antibody 画像

  • ab45415 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45415 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5min) HeLa cells at 5ug/ml.

  • All lanes : Anti-Caspase-14 antibody (ab45415) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate + blocking peptide

    Secondary
    anti-rabbit IgG

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

Anti-Caspase-14 antibody (ab45415) 使用論文

This product has been referenced in:
  • Asselin-Labat ML  et al. Gata-3 negatively regulates the tumor-initiating capacity of mammary luminal progenitor cells and targets the putative tumor suppressor caspase-14. Mol Cell Biol 31:4609-22 (2011). WB . Read more (PubMed: 21930782) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocytes)
Loading amount
20 µg
Specification
Keratinocytes
Gel Running Conditions
Reduced Denaturing (4~12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. HYOUNG JUNE KIM

Verified customer

投稿 Oct 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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