製品の概要

  • 製品名
    Caspase 12 Assay Kit (Fluorometric)
    Caspase 12 キット 製品一覧
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's Caspase 12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 12 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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  • 特記事項

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

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参考文献

This product has been referenced in:
  • Bulatovic I  et al. Sublethal caspase activation promotes generation of cardiomyocytes from embryonic stem cells. PLoS One 10:e0120176 (2015). Functional Studies . Read more (PubMed: 25763592) »
  • Mitra A  et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). Functional Studies . Read more (PubMed: 24464634) »

See all 2 Publications for this product

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