製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Caspase-12
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Dog, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-12 aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6UXS9
    (Peptide available as ab98317, ab98329, ab98329)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse and Rat Spleen tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

ターゲット情報

画像

  • All lanes : Anti-Caspase-12 antibody (ab87348) at 1 µg/ml

    Lane 1 : Spleen (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

プロトコール

参考文献

This product has been referenced in:
  • Marsili S  et al. Exclusion of the unfolded protein response in light-induced retinal degeneration in the canine T4R RHO model of autosomal dominant retinitis pigmentosa. PLoS One 10:e0115723 (2015). Read more (PubMed: 25695253) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Dog Cell lysate - whole cell (MDCK)
Specification
MDCK
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat podocyte)
Loading amount
30 µg
Specification
rat podocyte
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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