製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Caspase-12
  • 特異性
    This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
    Database link: O08736

  • ポジティブ・コントロール
    • WB: Human heart lysate. IHC-P: Human and rat heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 18621034
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

Anti-Caspase-12 antibody 画像

  • Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.

  • Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
    Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit IgG secondary antibody

    Predicted band size : 38-45 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.

  • All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

    Lane 1 : rat dorsal root ganglion
    Lane 2 : treated rat dorsal root ganglion

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-45 kDa


    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France

    The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

    See Abreview

  • Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.

Anti-Caspase-12 antibody (ab62484) 使用論文

This product has been referenced in:
  • Zhu G  et al. Endoplasmic reticulum stress mediates distinct impacts of sevoflurane on different subfields of immature hippocampus. J Neurochem 142:272-285 (2017). Read more (PubMed: 28444766) »
  • Wu Z  et al. Silencing of both ATF4 and PERK inhibits cell cycle progression and promotes the apoptosis of differentiating chondrocytes. Int J Mol Med 40:101-111 (2017). IHC ; Mouse . Read more (PubMed: 28498443) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2 cell line)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine gel)
Loading amount
50000 cells
Specification
HEPG2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 25 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100000 cells
Treatment
Bortezomib 0-20hours
Specification
MOLT4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 19 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2014

Thank you for contacting us.

The lab let me know the following:

Both antibodies should recognize the pro form and the fragment downstream of the K158/T159 cleavage site. ab62484 detects a band about 45kDa in human heart and ab62463...

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Thank you for calling Abcam.
I am sorry that the vial of ab62484 that you received was short and I am sending you another vial to make up for that. Your new order number is *.
If there is anything else I can help you with, please let me know.

Thank you for your inquiry.
I am happy to confirm that all these antibodies are tested and guaranteed to work with human samples.
You are correct that we do not have any reports yet about the use of these antibodies in muscle. I assume that i...

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Thank you for contacting us.

Usinga single antibody, the only way to distinguish between the pro and cleaved forms of Caspase 12 would be by the molecular weight in a western blot. The full length pro Caspase 12 will run at ˜42 kDa, while the...

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Thank you for your reply.

Regrettably, I canconfirm that we donotsell the specific blocking peptide for this antibody.

I am sorry we do not have the product to meet your requirements on this occasion. If you have any further quest...

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Thank you for your inquiry. I can confirm that the immunogen sequence is downstream of the cleavage site at approximately residue 120. This means the antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Dorsal root ganglion)
Loading amount
15 µg
Specification
Dorsal root ganglion
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Karine Thibault

Verified customer

投稿 Oct 13 2010

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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