製品の概要

  • 製品名Caspase 12 (active) FITC Staining Kit
    Caspase 12 キット 製品一覧
  • アッセイタイプEnzyme activity
  • 製品の概要

    The Caspase 12 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 12 in living cells. The assay utilizes the caspase 12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 12 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

  • アプリケーション適用あり: Functional Studies, Flow Cytmore details

製品の特性

  • 関連性Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF.
  • 別名
    • CASP12
    • CASP12P1
    • Caspase 12
    • Inactive caspase 12

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

プロトコール

Caspase 12 (active) FITC Staining Kit (ab65610) 使用論文

This product has been referenced in:
  • Ryan SD  et al. Neuronal dystonin isoform 2 is a mediator of endoplasmic reticulum structure and function. Mol Biol Cell 23:553-66 (2012). Read more (PubMed: 22190742) »

See 1 Publication for this product

Product Wall

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"