製品の概要

  • 製品名Anti-Caspase-1 antibody
    Caspase-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-1 aa 350 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P29466
    (Peptide available as ab91439, ab91439)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human spleen and lung tissue lysates as well as the following whole cell lysates: HeLa; A549; HepG2; CaCo2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 組織特異性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 antibody 画像

  • All lanes : Anti-Caspase-1 antibody (ab82563) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 50 kDa,87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

Anti-Caspase-1 antibody (ab82563) 使用論文

ab82563 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"