Anti-Caspase-1 抗体 [14F468] (ab14367)

製品の概要

  • 製品名Anti-Caspase-1 antibody [14F468]
    Caspase-1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14F468] to Caspase-1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-1 aa 371-390.
    Sequence:

    RKVRFSFEQPDGRAQMPTTE


    Database link: P29466

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab14367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa.
IHC-P 1/10 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 組織特異性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻訳後修飾The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 antibody [14F468] 画像

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human lung tissue labeling Caspase 1 with ab14367 at 5 ug/ml. In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human skin tissue labeling Caspase 1 with ab14367 at 5 ug/ml. Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded normal Human colon tissue labeling Caspase 1 with ab14367 at 5 ug/ml. Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human adenocarcinoma of the rectum tissue labeling Caspase 1 with ab14367 at 5 ug/ml. peroxidase-conjugate and DAB chromogen.

     

    Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20

    min followed by cooling at RT for 20 min.

  • Immunohistochemical analysis of paraffin embedded Human ovarian cancer tissue labeling Caspase 1 with ab14367 at 5 ug/ml.

     

     

  • Western blot analysis of Caspase-1 in HeLa cell lysate using ab14367 at 0.5 ug/ml.

    Western blot analysis of Caspase-1 in HeLa cell lysate using ab14367 at 0.5 ug/ml.

Anti-Caspase-1 antibody [14F468] (ab14367) 使用論文

ab14367 has not yet been referenced specifically in any publications.

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I am glad I could be of assistance. If you need any other help with setting up your assay, please let me know and I will be happy to help you further.

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Thank you for your enquiry. Below are some suggestions, please take a look at the online datasheets for more information regarding these antibodies. If you have any additional questions, please contact us again. Caspase 1 - The following antibodies ...

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