Anti-Casein Kinase 1 gamma 2 抗体 - C-terminal (ab70862)

製品の概要

  • 製品名Anti-Casein Kinase 1 gamma 2 antibody - C-terminal
    Casein Kinase 1 gamma 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Casein Kinase 1 gamma 2 - C-terminal
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Casein Kinase 1 gamma 2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Mouse muscle tissue lysate and human hepatocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50.
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
  • 組織特異性Testis.
  • 配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 1, gamma 2 antibody
    • Casein kinase I isoform gamma 2 antibody
    • Casein kinase I isoform gamma-2 antibody
    • CK1g2 antibody
    • CKI gamma 2 antibody
    • CKI-gamma 2 antibody
    • CSNK1G 2 antibody
    • Csnk1g2 antibody
    • KC1G2_HUMAN antibody
    see all

Anti-Casein Kinase 1 gamma 2 antibody - C-terminal 画像

  • Lanes 1 - 2 : Anti-Casein Kinase 1 gamma 2 antibody (ab102554)
    Lanes 3 - 4 : Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862) at 1/200 dilution
    Lanes 5 - 6 : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution

    Lane 1 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2
    Lane 2 : U2OS cells transfected with a plasmid expressing FLAG only
    Lane 3 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2
    Lane 4 : U2OS cells transfected with a plasmid expressing FLAG only
    Lane 5 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2
    Lane 6 : U2OS cells transfected with a plasmid expressing FLAG only

    Lysates/proteins at 90 µg per lane.

    Secondary
    HRP- conjugated Goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 47 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862) at 1/100 dilution + mouse muscle tissue lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 47 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical staining of formalin-fixed, paraffin-embedded human hepatocarcinoma with ab70862 at a 1/10 dilution, followed by HRP-conjugated secondary antibody and DAB staining.

Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862) 使用論文

ab70862 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"