Anti-Casein Kinase 1 gamma 2 抗体 (ab102554)

製品の概要

  • 製品名Anti-Casein Kinase 1 gamma 2 antibody
    Casein Kinase 1 gamma 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Casein Kinase 1 gamma 2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 359-408 (SKNQALNSTN GELNADDPTA GHSNAPITAP AEVEVADETK CCCFFKRRKR) of Human Casein Kinase 1 gamma 2 (NP_001310).

  • ポジティブ・コントロール
    • A549 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
  • 組織特異性Testis.
  • 配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 1, gamma 2 antibody
    • Casein kinase I isoform gamma 2 antibody
    • Casein kinase I isoform gamma-2 antibody
    • CK1g2 antibody
    • CKI gamma 2 antibody
    • CKI-gamma 2 antibody
    • CSNK1G 2 antibody
    • Csnk1g2 antibody
    • KC1G2_HUMAN antibody
    see all

Anti-Casein Kinase 1 gamma 2 antibody 画像

  • Anti-Casein Kinase 1 gamma 2 antibody (ab102554) at 1 µg/ml + A549 cell lysate at 10 µg

    Predicted band size : 47 kDa

Anti-Casein Kinase 1 gamma 2 antibody (ab102554) 使用論文

ab102554 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"