製品の概要

  • 製品名
    Anti-Casein Kinase 1 delta antibody
    Casein Kinase 1 delta 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Casein Kinase 1 delta
  • 特異性
    This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    DREERLRHSRNPATR

    from the internal region of the protein (Human)

  • ポジティブ・コントロール
    • HEK 293 cell lysate and lysates of Human Brain

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    Antibody detection limit dilution 1/64,000.

    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
    • 組織特異性
      Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
    • 関連疾患
      Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
    • 配列類似性
      Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated on serine and threonine residues.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Casein kinase I delta isoform antibody
      • Casein kinase I isoform delta antibody
      • CKI delta antibody
      • CKI-delta antibody
      • CKId antibody
      • CKIdelta antibody
      • CSNK 1D antibody
      • CSNK1D antibody
      • HCKID antibody
      • KC1D_HUMAN antibody
      • Protein kinase CK1 delta antibody
      see all

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    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK 293)
    Loading amount
    20 µg
    Specification
    HEK 293
    Treatment
    DMSO, 24 hours
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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