The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
機能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
組織特異性Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
関連疾患Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.
翻訳後修飾Autophosphorylated on serine and threonine residues.
ab10877 at 1/200 dilution staining Casein Kinase 1 delta in mouse skeletal muscle tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples from 4 to 6 week old BALB/c mice were immediately removed after killing and immersion fixed in Bouin's solution. Primary antibody was used for 40 minutes at RT. Specific antibody binding was visualised by the peroxidase reaction using DAB as substrate-chromogen.
Lin SH et al. Casein kinase 1 epsilon expression predicts poorer prognosis in low T-stage oral cancer patients. Int J Mol Sci15:2876-91 (2014).
Read more (PubMed: 24557581) »
Löhler J et al. Immunohistochemical characterisation of cell-type specific expression of CK1delta in various tissues of young adult BALB/c mice. PLoS One4:e4174 (2009).
Read more (PubMed: 19137063) »