Anti-Casein Kinase 1 alpha 抗体 (ab63373)

製品の概要

  • 製品名Anti-Casein Kinase 1 alpha antibody
    Casein Kinase 1 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Casein Kinase 1 alpha
  • アプリケーション適用あり: WB, ICC/IF, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Casein Kinase 1 alpha. Synthetic non-phosphopeptide (Human) from around the phosphorylation site of threonine 321 (AQTPPT)

  • ポジティブ・コントロール
    • HUVEC cell extract K562 cell extract HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • 配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Casein kinase 1 alpha 1 antibody
    • Casein kinase I isoform alpha antibody
    • CK1 antibody
    • CK1A antibody
    • CKI alpha antibody
    • CKI-alpha antibody
    • CKIa antibody
    • Clock regulator kinase antibody
    • Csnk1a1 antibody
    • Down regulated in lung cancer antibody
    • HLCDGP1 antibody
    • KC1A_HUMAN antibody
    • PRO2975 antibody
    see all

Anti-Casein Kinase 1 alpha antibody 画像

  • All lanes : Anti-Casein Kinase 1 alpha antibody (ab63373) at 1/500 dilution

    Lane 1 : HUVEC cell extract
    Lane 2 : K562 cell extract
    Lane 3 : Peptide blocking experiment, K562 Lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Immunofluorescence analysis of HeLa cells, using ab63373 at 1/500 dilution. Right hand panel was treated with immunising peptide.
  • Ab63373 staining human normal lung. Staining is localised to the cytoplasm (apical).
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Casein Kinase 1 alpha antibody (ab63373) 使用論文

ab63373 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"