製品の概要

  • 製品名Anti-Casein antibody [F20.14]
    Casein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F20.14] to Casein
  • 特異性This antibody does not react with alpha lactalbumin. This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • アプリケーション適用あり: IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名F20.14
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-Fr Use at an assay dependent concentration. Fix with acetone.

ターゲット情報

  • 機能Important role in the capacity of milk to transport calcium phosphate.
    Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • 組織特異性Mammary gland specific. Secreted in milk.
  • 配列類似性Belongs to the alpha-casein family.
  • 翻訳後修飾Not glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha casein antibody
    • alpha-S1-casein antibody
    • CASA antibody
    • CASA1_HUMAN antibody
    • Casein alpha s1 antibody
    • casein, alpha antibody
    • casein, alpha-S1, included antibody
    • Casoxin-D antibody
    • CSN1 antibody
    • CSN1S1 antibody
    • Csna antibody
    • MGC149368 antibody
    see all

Anti-Casein antibody [F20.14] 画像

  • ab47972 staining Casein in Human lactating breast tissue biopsy by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% normal serum containing 0.05% Tween for 30 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. A biotin-conjugated Donkey anti-Mouse IgG was used as the secondary antibody, diluted 1/250.

    See Abreview

Anti-Casein antibody [F20.14] (ab47972) 使用論文

This product has been referenced in:
  • Bonuccelli G  et al. The milk protein a-casein functions as a tumor suppressor via activation of STAT1 signaling, effectively preventing breast cancer tumor growth and metastasis. Cell Cycle 11:3972-82 (2012). Read more (PubMed: 23047602) »
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human lactating breast biopsy)
Specification Human lactating breast biopsy
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Normal serum containing 0.05% Tween as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"