製品の概要

  • 製品名Anti-CASC5 antibody
    CASC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CASC5
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 300 and 350 of human CASC5 using the numbering given in entry NP_733468.2

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or 293T cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.
  • 細胞内局在Acrosome. Nucleus.
  • 参照データベース
  • 別名
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    • Cancer susceptibility candidate 5 antibody
    • Cancer susceptibility candidate gene 5 protein antibody
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    • CT29 antibody
    • D40 antibody
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    • hSpc105 antibody
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    • Kinetochore null 1 homolog antibody
    • Kinetochore null protein 1 antibody
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    see all

Anti-CASC5 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CASC5 with ab70537 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CASC5 antibody (ab70537) at 1/5000 dilution

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg

    Developed using the ECL technique

    Predicted band size : 265 kDa
    Observed band size : >300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.

Anti-CASC5 antibody (ab70537) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Nov 17 2015

Thank you for your recent enquiries.

I am pleased to provide you with a copy of the western blot protocol used to test this antibody. Please note as previously discussed that this would be a guideline protocol only, and may require some furt...

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Thank you for your telephone call this morning. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forW...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"