製品の概要

  • 製品名Anti-CASC5 antibody
    CASC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CASC5
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 300 and 350 of human CASC5 using the numbering given in entry NP_733468.2

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or 293T cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.
  • 細胞内局在Acrosome. Nucleus.
  • 参照データベース
  • 別名
    • Protein D40/AF15q14 antibody
    • AF15Q14 antibody
    • ALL1 fused gene from chromosome 15q14 antibody
    • Blinkin bub linking kinetochore protein antibody
    • Cancer susceptibility candidate 5 antibody
    • Cancer susceptibility candidate gene 5 protein antibody
    • Cancer/testis antigen 29 antibody
    • CT29 antibody
    • D40 antibody
    • hKNL 1 antibody
    • hSpc105 antibody
    • KIAA1570 antibody
    • Kinetochore null 1 homolog antibody
    • Kinetochore null protein 1 antibody
    • KNL1 antibody
    see all

Anti-CASC5 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CASC5 with ab70537 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CASC5 antibody (ab70537) at 1/5000 dilution

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg

    Developed using the ECL technique

    Predicted band size : 265 kDa
    Observed band size : >300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • 1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.

Anti-CASC5 antibody (ab70537) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Nov 17 2015

Thank you for your recent enquiries.

I am pleased to provide you with a copy of the western blot protocol used to test this antibody. Please note as previously discussed that this would be a guideline protocol only, and may require some furt...

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Thank you for your telephone call this morning. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forW...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"